منابع مشابه
Radioimmunoassay of Benzoylecgonine in Blood and Urine
A sensitive RIA for benzoylecgonine in body fluids is described. The label is radioiodinated p-hydroxybenzoylecgonine. The antiserum from an Emit-dau cocaine metabolite kit (Syva) is used at a 1:600 dilution. The standards are benzoylecgonine in buffer. PEG-6000 is used to separate the bound and free fractions. Cocaine, ecgonine, and methylecgonine have cross-reactivities of 2%, 1%, and 0.1%, r...
متن کاملRadioimmunoassay of 2-hydroxyestrone in urine.
Catechol estrogens such as 2-OH estrone are interesting estrogen metabolites formed in several human tissues and excreted in urine. We developed and thoroughly validated a radioimmunoassay for urinary 2-OH estrone that has several advantages over published RIAs. Because we developed a relatively specific antiserum, we did not include a preliminary chromatographic step to eliminate cross-reactin...
متن کاملRadioimmunoassay of insulin in serum and plasma.
Paired serum and plasma samples from subjects were analyzed to determine if there was a difference in the insulin content of serum and plasma. Although there was no difference in the serum and plasma insulin concentrations in the samples as a group, there were differences in the serum and plasma insulin concentrations in individual subjects. Recovery of radioimmunoassayable insulin from clotted...
متن کاملRadioimmunoassay of free light chains of immunoglobulins in urine.
We describe radioimmunoassays for kappa and lambda light chains of immunoglobulins in urine. The assays, which involve antisera to free light chains, were sufficiently sensitive to measure the light chains in unconcentrated urine from healthy subjects. The usefulness of the assays in clinical practice is illustrated by measurements of light chain excretion by patients, including serial studies ...
متن کاملRadioimmunoassay of methaqualone and its monohydroxy metabolites in urine.
A commercial radioimmunoassay kit was evaluated for efficacy in detecting methaqualone or its metabolites in urine of persons receiving this drug. The drug and its unconjugated 3'- and 4'-monohydroxy metabolites could be detected equally well. The unconjugated alpha-monohydroxy metabolite was about 80% as reactive and the unconjugated 6-monohydroxy metabolite reacted only very weakly. Quantitat...
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ژورنال
عنوان ژورنال: Diabetologia
سال: 1967
ISSN: 0012-186X,1432-0428
DOI: 10.1007/bf01228082